Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia, 1(2), pp. Seleksi bakteri asam laktat sebagai penghasil enzim protease. Computational and Structural Biotechnology Journal, 2(3), pp. Technology Prospecting on Enzymes: Application, Marketing and Engineering. Arabian Journal for Science and Engineering, 41(6), pp. Purification and Characterization of a Membrane-Unbound Highly Thermostable Metalloprotease from Aeromonas Caviae. African Journal of Microbiology Research, 5(7), pp. Production optimization of an extracellular cold-active alkaline protease from Stenotrophomonas maltophilia MTCC 7528 and its application in detergent industry. Canadian Journal Microbiology, 55(11), pp. Cold-active extracellular alkaline protease from an alkaliphilic Stenotrophomonas maltophilia: production of enzyme and its industrial applications. Applied Microbiology and Biotechnology, 59(1), pp. Bacterial alkaline proteases: molecular approaches and industrial applications. Journal of Industrial Microbiology & Biotechnology, 35(2), pp. Purification and stability characteristics of an alkaline serine protease from a newly isolated Haloalkaliphilic bacterium sp. California USA.ĭodia, M.S., Rawal, C.M., Bhimani, H.G., Joshi, R.H., Khare, S.K.
Effects of temperature and incubation time on thein vitroexpression of proteases, phospholipases,lipases and DNases by different species of Trichosporon. Purification and characterization of an oxidation- stable, thiol-dependent serine alkaline protease from Bacillus mojavensis. Identification of proteases that regulate erythrocyte rupture by the malaria parasite Plasmodium falciparum. Grainger M., Phillips, C.I., Powers, J.C. The addition with ion Mn²+, K+, Na+ and Cu 2+ could act as inhibitors that might reduced the activity of the enzyme.Īkhdiya, A., 2003.Isolasi Bakteri Penghasil Enzim Protease Alkalin Termostabil. The addition of Ca2+ could activated its enzyme activity at the amount of 424.33U/mL, while without addition of the ion its activity was 400.29 U/mL. The enzyme has continued to its activity at pH 8 (419.68 U/mL) and maintained its stability at 398.22 U/mL with activities decreased to 94.87%, while its activity at 60☌ was 519.86 U/mL and could maintain its stability at 419.58 U/ mL, the activity decreased to 74.75%. We analyzed its proteolic activity against some effects of the incubation period, pH, temperatures and addition of monovalent and divalent metal ionsquantitatively using a spectrophotometer at ? 280 nm.The results showed that the optimum activity after incubation for two days was 315.88 U/ mL. Protease activity of the bacterial isolate was qualitatively determined by formation of a clear zone surrounded their colonies on media containing skim milk (1%). isolated from Mount Bromo, East Java in producing protease. The purpose of this study was to investigate the ability of Stenotrophomonas sp. These hydrolytic enzyme are efficiently involved in the food industry to increase the nutritional value, digestibility, palatability, flavour and reducing allergenic compounds as well as in the management of domestic and industrial wastes.
Microbial Proteases have the potency to be applied in industries such as detergents, skins, silver recovery, dairy, baking, beverages and pharmaceutical industries. Protease is an enzyme that can hydrolyze protein into simpler compounds, i.e peptides and amino acids.
0 kommentar(er)
